Effects of processing techniques on the forensic dna analysis of human skeletal remains

Human remains processed by forensic anthropologists may potentially be used for
Genetic analysis. Therefore, the condition of the deoxyribonucleic acid (DNA) in
Processed remains may become an issue for future analysis. Processing techniques
Employed by anthropologists are highly variable and scanning electron microscopy
Reveals significant alterations to the bone surface depending upon the technique
Used. Such damage to the bone indicates differences may exist in quality and
Quantity of DNA extracted. This study assessed how five processing procedures
Used by major forensic anthropology laboratories around the country affects the
Amounts of DNA extracted from human rib bones and the subsequent DNA analysis.
The DNA was analyzed using the short tandem repeat (STR) locus CSF1PO and
Amelogenin. The findings indicate processing procedures used by forensic
Anthropologists do not adversely affect DNA analysis but prolonged exposure to
Heat during processing may decrease the yield of information from the DNA.



Effects of processing techniques on the forensic dna analysis of human skeletal remains